William & Mary

Molecular Core Facility

DNA HelixThe Molecular Core Facility (CoreLab) assists investigators whose research projects require the use of molecular techniques such as DNA sequencing, fragment analysis, real-time PCR, quantification and quality assessment of RNA, DNA and proteins.

CoreLab services are available to all faculty members of the Biology Department. Students whose research projects require use of CoreLab instrumentation are trained in principles of operation and maintenance of instruments by CoreLab personnel.

Standard (Sanger method) sequencing is conducted with Applied Biosystems, Inc. (ABI) technology supported by the ABI 3500 Genetic Analyzer. Optional sequence data analysis can be performed with the use of ABI Variant Reporter software.

CoreLab offers the technology to conduct various genotyping/fragment analysis protocols, supported by ABI GeneMapper software; and assists with the development and use of real-time PCR technology, with two platforms available: ABI StepOne and Stratagene Mx3005P qPCR instruments.

CoreLab also offers an Agilent Bioanalyzer platform for a wide range of applications with its lab-on-a-chip technology: high resolution and quantification of DNA and RNA, and protein analysis.

PCR optimizing experiments can be conducted in CoreLab with the use of ABI Veriti thermocycler - a true temperature gradient with 8 custom temperature settings.

The latest addition to the CoreLab: a state-of-the-art Ion Torrent Personal Genome Sequencer opens a wide array of cutting-edge research possibilities.

To request CoreLab services, please contact Lidia Epp, Integrated Science Center, Room 3034, 221-2086, [[lmepp]]

DNA Sequencing Protocol [doc]

Sequencing Order Form [doc]