Molecular Core Facility

DNA HelixCoreLab services are available to all faculty members of the Biology Department. Students whose research projects require use of CoreLab instrumentation are trained in principles of operation and maintenance of instruments by CoreLab personnel.

Standard (Sanger method) sequencing is conducted with Applied Biosystems, Inc. (ABI) technology supported by the ABI 3500 Genetic Analyzer. Optional sequence data analysis can be performed with the use of ABI Variant Reporter software.

CoreLab offers the technology to conduct various genotyping/fragment analysis protocols, supported by ABI GeneMapper software.

There are two ABI real-time PCR instruments available: StepOne (48well format and 5 fluorescent dye capability) and StepOne Plus (96 well format and 7 fluorescent dye spectrum). Both instruments support real-time quantitative assays, gene expression assays, genotyping and presence-absence experiments.

CoreLab also offers an Agilent Bioanalyzer platform for a wide range of applications with its lab-on-a-chip technology: high resolution and quantification of DNA and RNA, and protein analysis.

PCR optimizing experiments can be conducted in CoreLab with the use of ABI Veriti thermocycler - a true temperature gradient with 8 custom temperature settings.

To request CoreLab services, please contact Lidia Epp, Integrated Science Center, Room 3034, 221-2086, [[lmepp]]

DNA Sequencing Protocol [doc]

Sequencing Order Form [doc]